LOCUS       HUMGAPDHG    5378 bp    DNA             PRI       15-NOV-1994
DEFINITION  Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene,
            complete cds.
ACCESSION   J04038
VERSION     J04038.1  GI:182980
KEYWORDS    glyceraldehyde-3-phosphate dehydrogenase.
SOURCE      Human DNA.
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Mammalia;
            Eutheria; Primates; Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5378; 1 to 5378)
  AUTHORS   Ercolani,L., Florence,B., Denaro,M. and Alexander,M.
  TITLE     Isolation and complete sequence of a functional human
            glyceraldehyde-3-phosphate dehydrogenase gene
  JOURNAL   J. Biol. Chem. 263 (30), 15335-15341 (1988)
  MEDLINE   89008430
REFERENCE   2  (bases 650 to 1157)
  AUTHORS   Nasrin,N., Ercolani,L., Denaro,M., Kong,X.F., Kang,I. and
            Alexander,M.
  TITLE     An insulin response element in the glyceraldehyde-3-phosphate
            dehydrogenase gene binds a nuclear protein induced by insulin in
            cultured cells and by nutritional manipulations in vivo
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 87 (14), 5273-5277 (1990)
  MEDLINE   90319094
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /map="12p13"
     misc_signal     650..729
                     /note="insulin response element A [2]"
     misc_signal     729..867
                     /note="insulin response element B [2]"
     old_sequence    877..879
                     /citation=[1]
     CAAT_signal     884..892
     misc_signal     1049..1071
                     /note="insulin response element A [2]"
     TATA_signal     1108..1113
     prim_transcript 1138..4993
                     /note="GAPDH mRNA and introns"
     exon            1138..1189
                     /gene="GAPD"
                     /note="glyceraldehyde-3-phosphate dehydrogenase,  (first
                     expressed exon) (EC 1.2.2.12)"
     intron          1190..1429
                     /note="GAPDH intron A"
     misc_feature    1447..1456
                     /note="Kozak consensus sequence; putative"
     gene            join(1138..1189,1430..1481,3116..3215,3306..3412,3542..3632,
                     3723..3838,3931..4012,4206..4618,4723..4792)
                     /gene="GAPD"
     CDS             join(1453..1481,3116..3215,3306..3412,3542..3632,
                     3723..3838,3931..4012,4206..4618,4723..4792)
                     /gene="GAPD"
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
                     /codon_start=1
                     /db_xref="GDB:G00-119-249"
                     /protein_id="AAA53191.1"
                     /db_xref="GI:182981"
                     /translation="MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMV
                     YMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVF
                     TTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPL
                     AKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVG
                     KVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGY
                     TEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASK
                     E"
     exon            1430..1481
                     /gene="GAPD"
                     /number=2
     intron          1482..3115
                     /note="GAPDH intron B"
     exon            3116..3215
                     /gene="GAPD"
     intron          3216..3305
                     /note="GAPDH intron C"
     exon            3306..3412
                     /gene="GAPD"
                     /number=4
     intron          3413..3541
                     /note="GAPDH intron D"
     exon            3542..3632
                     /gene="GAPD"
                     /number=5
     intron          3633..3722
                     /note="GAPDH intron E"
     exon            3723..3838
                     /gene="GAPD"
                     /number=6
     intron          3839..3930
                     /note="GAPDH intron F"
     exon            3931..4012
                     /gene="GAPD"
                     /number=7
     intron          4013..4205
                     /note="GAPDH intron G"
     exon            4206..4618
                     /gene="GAPD"
                     /number=8
     intron          4619..4722
                     /note="GAPDH intron H"
     exon            4723..>4792
                     /gene="GAPD"
                     /note="glyceraldehyde-3-phosphate dehydrogenase"
                     /number=9
BASE COUNT      994 a   1642 c   1627 g   1115 t
ORIGIN      
        1 ggatcccctg ctgggagggg gcaggggacc tgttcccacc gtgtgcccaa gacctctttt
       61 cccacttttt ccctcttctt gactcaccct gccctcaata tcccccggcg cagcagtgaa
      121 agggagtccc tggctcctgg ctcgcctgca cgtcccaggg cggggaggga cttccgccct
      181 cacgtcccgc tcttcgcccc aggctggatg gaatgaaagg cacactgtct ctctccctag
      241 gcagcacagc ccacaggttt caggagtgcc tttgtgggag gcctctgggc ccccaccagc
      301 catcctgtcc tccgcctggg gccccagccc ggagagagcc gctggtgcac acagggccgg
      361 gattgtctgc cctaattatc aggtccaggc tacagggctg caggacatcg tgaccttccg
      421 tgcagaaacc tccccctccc cctcaagccg cctcccgagc ctccttcctc tccaggcccc
      481 cagtgcccag tgcccagtgc ccagcccagg cctcggtccc agagatgcca ggagccagga
      541 gatggggagg gggaagtggg ggctgggaag gaaccacggg cccccgcccg agcccatggg
      601 cccctcctag gcctttgcct gagcagaccg gtgtcactac cgcagagcct cgaggagaag
      661 ttccccaact ttcccgcctc tcagcctttg aaagaaagaa aggggagggg gcaggccgcg
      721 tgcagccgcg agcggtgctg ggctccggct ccaattcccc atctcagtcg ttcccaaagt
      781 cctcctgttt catccaagcg tgtaagggtc cccgtccttg actccctagt gtcctgctgc
      841 ccacagtcca gtcctgggaa ccagcaccga tcacctccca tcgggccaat ctcagtccct
      901 tccccctacg tcggggccca cacgctcggt gcgtgcccag ttgaaccagg cggctgcgga
      961 aaaaaaaaag cggggagaaa gtagggcccg gctactagcg gttttacggg cgcacgtagc
     1021 tcaggcctca agaccttggg ctgggactgg ctgagcctgg cgggaggcgg ggtccgagtc
     1081 accgcctgcc gccgcgcccc cggtttctat aaattgagcc cgcagcctcc cgcttcgctc
     1141 tctgctcctc ctgttcgaca gtcagccgca tcttcttttg cgtcgccagg tgaagacggg
     1201 cggagagaaa cccgggaggc tagggacggc ctgaaggcgg caggggcggg cgcaggccgg
     1261 atgtgttcgc gccgctgcgg ggtgggcccg ggcggcctcc gcattgcagg ggcgggcgga
     1321 ggacgtgatg cggcgcgggc tgggcatgga ggcctggtgg gggaggggag gggaggcgtg
     1381 tgtgtcggcc ggggccacta ggcgctcact gttctctccc tccgcgcagc cgagccacat
     1441 cgctcagaca ccatggggaa ggtgaaggtc ggagtcaacg ggtgagttcg cgggtggctg
     1501 gggggccctg ggctgcgacc gcccccgaac cgcgtctacg agccttgcgg gctccgggtc
     1561 tttgcagtcg tatgggggca gggtagctgt tccccgcaag gagagctcaa ggtcagcgct
     1621 cggacctggc ggagccccgc acccaggctg tggcgccctg tgcagctccg cccttgcggc
     1681 gccatctgcc cggagcctcc ttcccctagt ccccagaaac aggaggtccc tactcccgcc
     1741 cgagatcccg acccggaccc ctaggtgggg gacgctttct ttcctttcgc gctctgcggg
     1801 gtcacgtgtc gcagaggagc ccctccccca cggcctccgg caccgcaggc cccgggatgc
     1861 tagtgcgcag cgggtgcatc cctgtccgga tgctgcgcct gcggtagagc ggccgccatg
     1921 ttgcaaccgg gaaggaaatg aatgggcagc cgttaggaaa gcctgccggt gactaaccct
     1981 gcgctcctgc ctcgatgggt ggagtcgcgt gtggcgggga agtcaggtgg agcgaggcta
     2041 gctggcccga tttctcctcc gggtgatgct tttcctagat tattctctgg taaatcaaag
     2101 aagtgggttt atggaggtcc tcttgtgtcc cctccccgca gaggtgtggt ggctgtggca
     2161 tggtgccaag ccgggagaag ctgagtcatg ggtagttgga aaaggacatt tccaccgcaa
     2221 aatggcccct ctggtggtgg ccccttcctg cagcggctca cctcacggcc ccgcccttcc
     2281 cctgccagcc tagcgttgac ccgaccccaa aggccaggct gtaaatgtca ccgggaggat
     2341 tgggtgtctg ggcgcctcgg ggaacctgcc cttctcccca ttccgtcttc cggaaaccag
     2401 atctccaccg caccctggtc tgaggtctga ggttaaatat agctgctgac ctttctgtag
     2461 ctgggggcct gggctggggc tctctcccat cccttctccc cacacacatg cacttacctg
     2521 tgctcccact cctgatttct ggaaaagagc taggaaggac aggcaacttg gcaaatcaaa
     2581 gccctgggac tagggggtta aaatacagct tcccctcttc ccacccgccc cagtctctgt
     2641 cccttttgta ggagggactt agagaagggg tgggcttgcc ctgtccagtt aatttctgac
     2701 ctttactcct gccctttgag tttgatgatg ctgagtgtac aagcgttttc tccctaaagg
     2761 gtgcagctga gctaggcagc agcaagcatt cctggggtgg catagtgggg tggtgaatac
     2821 catgtacaaa gcttgtgccc agactgtggg tggcagtgcc cacatggccg cttctcctgg
     2881 aagggcttcg tatgactggg ggtgttgggc agccctggag ccttcagttg cagccatgcc
     2941 ttaagccagg ccagcctggc agggaagctc aagggagata aaattcaacc tcttgggccc
     3001 tcctgggggt aaggagatgc tgcattcgcc ctcttaatgg ggaggtggcc tagggctgct
     3061 cacatattct ggaggagcct cccctcctca tgccttcttg cctcttgtct cttagatttg
     3121 gtcgtattgg gcgcctggtc accagggctg cttttaactc tggtaaagtg gatattgttg
     3181 ccatcaatga ccccttcatt gacctcaact acatggtgag tgctacatgg tgagccccaa
     3241 agctggtgtg ggaggagcca cctggctgat gggcagcccc ttcataccct cacgtattcc
     3301 cccaggttta catgttccaa tatgattcca cccatggcaa attccatggc accgtcaagg
     3361 ctgagaacgg gaagcttgtc atcaatggaa atcccatcac catcttccag gagtgagtgg
     3421 aagacagaat ggaagaaatg tgctttgggg aggcaactag gatggtgtgg ctcccttggg
     3481 tatatggtaa ccttgtgtcc ctcaatatgg tcctgtcccc atctcccccc caccccggta
     3541 ggcgagatcc ctccaaaatc aagtggggcg atgctggcgc tgagtacgtc gtggagtcca
     3601 ctggcgtctt caccaccatg gagaaggctg gggtgagtgc aggagggccc gcgggagggg
     3661 aagctgactc agccctgcaa aggcaggacc cgggttcata actgtctgct tctctgctgt
     3721 aggctcattt gcagggggga gccaaaaggg tcatcatctc tgccccctct gctgatgccc
     3781 ccatgttcgt catgggtgtg aaccatgaga agtatgacaa cagcctcaag atcatcaggt
     3841 gaggaaggca gggcccgtgg agaagcggcc agcctggcac cctatggaca cgctcccctg
     3901 acttgcgccc cgctccctct ttctttgcag caatgcctcc tgcaccacca actgcttagc
     3961 acccctggcc aaggtcatcc atgacaactt tggtatcgtg gaaggactca tggtatgaga
     4021 gctggggaat gggactgagg ctcccacctt tctcatccaa gactggctcc tccctgctgg
     4081 ggctgcgtgc aaccctgggg ttgggggttc tggggactgg ctttcccata atttcctttc
     4141 aaggtgggga gggaggtaga ggggtgatgt ggggagtacg ctgcagggcc tcactccttt
     4201 tgcagaccac agtccatgcc atcactgcca cccagaagac tgtggatggc ccctccggga
     4261 aactgtggcg tgatggccgc ggggctctcc agaacatcat ccctgcctct actggcgctg
     4321 ccaaggctgt gggcaaggtc atccctgagc tgaacgggaa gctcactggc atggccttcc
     4381 gtgtccccac tgccaacgtg tcagtggtgg acctgacctg ccgtctagaa aaacctgcca
     4441 aatatgatga catcaagaag gtggtgaagc aggcgtcgga gggccccctc aagggcatcc
     4501 tgggctacac tgagcaccag gtggtctcct ctgacttcaa cagcgacacc cactcctcca
     4561 cctttgacgc tggggctggc attgccctca acgaccactt tgtcaagctc atttcctggt
     4621 atgtggctgg ggccagagac tggctcttaa aaagtgcagg gtctggcgcc ctctggtggc
     4681 tggctcagaa aaagggccct gacaactctt ttcatcttct aggtatgaca acgaatttgg
     4741 ctacagcaac agggtggtgg acctcatggc ccacatggcc tccaaggagt aagacccctg
     4801 gaccaccagc cccagcaaga gcacaagagg aagagagaga ccctcactgc tggggagtcc
     4861 ctgccacact cagtccccca ccacactgaa tctcccctcc tcacagttgc catgtagacc
     4921 ccttgaagag gggaggggcc tagggagccg caccttgtca tgtaccatca ataaagtacc
     4981 ctgtgctcaa ccagttactt gtcctgtctt attctagggt ctggggcaga ggggagggaa
     5041 gctgggcttg tgtcaaggtg agacattctt gctggggagg gacctggtat gttctcctca
     5101 gactgagggt agggcctcca aacagccttg cttgcttcga gaaccatttg cttcccgctc
     5161 agacgtcttg agtgctacag gaagctggca ccactacttc agagaacaag gccttttcct
     5221 ctcctcgctc cagtcctagg ctatctgctg ttggccaaac atggaagaag ctattctgtg
     5281 ggcagcccca gggaggctga caggtggagg aagtcagggc tcgcactggg ctctgacgct
     5341 gactggttag tggagctcag cctggagctg agctgcag
//



