Biology seminar: Genetic engineering and biotechnology
BIO 425
Instructor: Jim Lund


Biotech timeline

Papers can be found searching at these sites:

NCBI Pubmed

Google Scholar
Older publications can be found in the library.

The commercial, cloned banana is getting overrun by fungus and will go extinct. Popular science article, Snopes urban legend article.

Week Topic Paper(s) Monday Presenter Tuesday Presenter
1 ELISA (Enzyme-linked immunosorbent assay), the assay used in pregnancy tests Immunoassay using antigen-enzyme conjugates. Van Weemen BK, Schuurs AH (1971). FEBS Letters 15 (3): 232–6.
Related information:

ELISA (wikipedia)

How a pregnancy test works
2 DNA cloning (Making recombinant human insulin) A bacterial clone synthesizing proinsulin. Villa-Komaroff L, Efstratiadis A, Broome S, Lomedico P, Tizard R, Naber SP, Chick WL, Gilbert W. Proc Natl Acad Sci U S A. 1978 August; 75(8): 3727–3731.
Additional reading:
Expression in Escherichia coli of chemically synthesized genes for human insulin. Goeddel DV, Kleid DG, Bolivar F, Heyneker HL, Yansura DG, Crea R, Hirose T, Kraszewski A, Itakura K, Riggs AD. Proc Natl Acad Sci U S A. 1979 Jan;76(1):106-10.
Review of initial cloning steps: review
Construction of Biologically Functional Bacterial Plasmids In Vitro. Stanley N. Cohen, Annie C. Y. Chang, Herbert W. Boyer, and Robert B. Helling. Proc Natl Acad Sci U S A. 1973 November; 70(11): 3240–3244.
Andrew L. Scott K.
3 Monoclonal antibodies Continuous cultures of fused cells secreting antibody of predefined specificity. Köhler G, Milstein C. Nature 256, 495 - 497 (1975).
Additional reading:
Monoclonal Antibodies: Producing Magic Bullets by Somatic Cell Hybridization. David H. Margulie. The Journal of Immunology, 2005, 174: 2451-2452. HTML, PDF
Quenio F. Lindsey C.
4 Preimplantation genetic diagnosis Birth of a normal girl after in vitro fertilization and preimplantation diagnostic testing for cystic fibrosis. AH Handyside, JG Lesko, JJ Tarin, RM Winston, and MR Hughes. NEJM Volume 327:905-909
Abstract & refs, PDF
Additional reading:
Pregnancy After Embryo Biopsy and Coamplification of DNA From X and Y Chromosomes
Jamie A. Grifo; Ya Xu Tang; Jacques Cohen; Fred Gilbert; Mrinal K. Sanyal; Zev Rosenwaks
JAMA, Vol. 268, Issue 6, 727-729, August 12, 1992
Abstract, PDF
Justin D. Andrew W.
5 Sanger DNA sequencing DNA sequencing with chain-terminating inhibitors F. Sanger, S. Nicklen, and A. R. Coulson PNAS December 1, 1977 v74:12, 5463-5467.
Jonathan P. Lacey B.
6 Mouse transgenics Genetic transformation of mouse embryos by microinjection of purified DNA. J W Gordon, G A Scangos, D J Plotkin, J A Barbosa, and F H Ruddle. Proc Natl Acad Sci U S A. 1980 December; 77(12): 7380–7384.
Micheal M. Sara C.
7 Mouse embryonic stem cell technology Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Simon Thompson, Alan R. Clarke, Angela M. Pow, Martin L. Hooper, and David W. Melton. Cell Volume 56, Issue 2, 27 January 1989, Pages 313-321.
Abstract & links, PDF
Jason B. Satrio H.
8 Stem cell isolation Purification and Characterization of Mouse Hematopoietic Stem Cells. Gerald J. Spangrude, Shelly Heimfeld and Irving L. Weissman. Science, New Series, Vol. 241, No. 4861 (Jul. 1, 1988), pp. 58-62
Kelly A. Jemma K.
9 Mammalian cloning (Dolly the sheep) Sheep cloned by nuclear transfer from a cultured cell line. Campbell KH, McWhir J, Ritchie WA, Wilmut I. Nature. 1996 Mar 7;380(6569):64-6.
  Courtney C.
10 DNA microarrays Parallel human genome analysis: microarray-based expression monitoring of 1000 genes. M Schena, D Shalon, R Heller, A Chai, P O Brown, and R W Davis. PNAS October 1, 1996 vol. 93 no. 20 10614-10619
Abstract & refs, PDF
Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Schena M, Shalon D, Davis RW, Brown PO. Science (1995) 270: 467–470.
Tuoxin C. Micheal C.
11 Next gen DNA sequencing Genome Sequencing in Open Microfabricated High Density Picoliter Reactors.
Marcel Margulies, Michael Egholm, William E. Altman, Said Attiya, Joel S. Bader, Lisa A. Bemben, Jan Berka, Michael S. Braverman, Yi-Ju Chen, Zhoutao Chen, Scott B. Dewell, Lei Du, Joseph M. Fierro, Xavier V. Gomes, Brian C. Goodwin, Wen He, Scott Helgesen, Chun He Ho, Gerard P. Irzyk, Szilveszter C. Jando, Maria L.I. Alenquer, Thomas P. Jarvie, Kshama B. Jirage, Jong-Bum Kim, James R. Knight, Janna R. Lanza, John H. Leamon, Steven M. Lefkowitz, Ming Lei, Jing Li, Kenton L. Lohman, Hong Lu, Vinod B. Makhijani, Keith E. McDade, Michael P. McKenna, Eugene W. Myers,3 Elizabeth Nickerson, John R. Nobile, Ramona Plant, Bernard P. Puc, Michael T. Ronan, George T. Roth, Gary J. Sarkis, Jan Fredrik Simons, John W. Simpson, Maithreyan Srinivasan, Karrie R. Tartaro, Alexander Tomasz,4 Kari A. Vogt, Greg A. Volkmer, Shally H. Wang, Yong Wang, Michael P. Weiner, Pengguang Yu, Richard F. Begley, and Jonathan M. Rothberg.
Nature. 2005 September 15; 437(7057): 376–380.

Additional reading:
GENE SEQUENCING: The Race for the $1000 Genome.
Robert F. Service.
Science 17 March 2006: Vol. 311. no. 5767, pp. 1544 - 1546.

  Katie D.
12 Stem cell treatment Autologous Nonmyeloablative Hematopoietic Stem Cell Transplantation in Newly Diagnosed Type 1 Diabetes Mellitus.
JúC. Voltarelli, MD, PhD; Carlos E. B. Couri, MD, PhD; Ana B. P. L. Stracieri, MD, PhD; Maria C. Oliveira, MD, MSc; Daniela A. Moraes, MD; Fabiano Pieroni, MD, PhD; Marina Coutinho, MD, MSc; Kelen C. R. Malmegrim, PhD; Maria C. Foss-Freitas, MD, PhD; Belinda P. Simõ MD, PhD; Milton C. Foss, MD, PhD; Elizabeth Squiers, MD; Richard K. Burt, MD. JAMA. 2007;297:1568-1576.
Thomas V. Dana B.
13 Targeting tumor cells Cell surface expression of the stress response chaperone GRP78 enables tumor targeting by circulating ligands.
Marco A. Arap, Johanna Lahdenranta, Paul J. Mintz, Amin Hajitou, Álvaro S. Sarkis, Wadih Arap, and Renata Pasqualini.
Cancer Cell, Volume 6, Issue 3, 275-284, 1 September 2004

  Seth R.
14 SNP studies Coat Variation in the Domestic Dog Is Governed by Variants in Three Genes.
Edouard Cadieu, Mark W. Neff, Pascale Quignon, Kari Walsh, Kevin Chase, Heidi G. Parker, Bridgett M. VonHoldt, Alison Rhue, Adam Boyko, Alexandra Byers, Aaron Wong, Dana S. Mosher, Abdel G. Elkahloun, Tyrone C. Spady, Catherine André, K. Gordon Lark, Michelle Cargill,,* Carlos D. Bustamante, Robert K. Wayne, Elaine A. Ostrander.
Science 2 October 2009: Vol. 326. no. 5949, pp. 150 - 153.

not for Monday's class  

Monoclonal antibodies
Gene therapy